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Revision as of 13:16, 7 October 2022

Cas12g

Part Description

RNA-guided Among its primary targets are single-stranded RNA substrates, Cas12g is a ribonuclease. Comparing it to other Cas12 proteins that have been found so far, CRISPS-Cas12g selectively detects RNA substrates, making it a potentially useful platform for transcriptome editing and diagnostics. While guided RNAs fold into a "F" shape that is primarily identified by the Rec lobes, a bilobed structure of Cas12g displays a tiny NUC2 and REC2 domain. To change the conformation of the REC and NUC lobes and activate Cas12g, target RNA and crRNA guide combine to form a duplex that is inserted into the cavity in the middle of the structure.

Usage

Cas12g is a RNA-guided protein and differs from other Cas12 proteins by targeting a single strand RNA substrates Making it a potent platform for post transcription modification so we use to control PAH and beta-galactosidase expression as it cleaves the mRNA of PAH and beta-galactosidase at specific site without the need to recognize the PAM Sequence distinguishing it from other Cas proteins preventing the translation PAH and beta-galactosidase just in case of over expression of them or high level of tyrosine and absence of L7Ae as shown in figure 1.

Figure(1) Shows an SBOL demonstrating the usage of cas12g in our whole cel-based biosensor

References

Sequence and Features