Difference between revisions of "Part:BBa K4121002"

Revision as of 12:56, 7 October 2022

pPGK1 We paid attention to an existing basic part in the iGEM part library, the S. Cerevisiae promoter PGK1, and tried to improve it. As we know, an excessively long target sequence is not conducive to molecular experiments, sequencing and bioinformatics analysis, which will cause a lot of trouble to the experimenters. At present, the length of the PGK1 promoter in iGEM component library is 1497bp. We truncate 1100bp, 800bp, 600bp, 400bp or even 200bp in the existing sequence of PGK1 as the promoter sequence, and then use the red protein mCherry characterizes the startup strength of the PGK1 promoter of different lengths, trying to find the optimal PGK1 promoter with the same strength and shorter sequence compared the original promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K4121002 short</partinfo>
-pPGK1 is a promoter in Saccharomyces cerevisiae, and has relatively strong expression strength.
+We paid attention to an existing basic part in the iGEM part library, the S. Cerevisiae promoter PGK1, and tried to improve it. As we know, an excessively long target sequence is not conducive to molecular experiments, sequencing and bioinformatics analysis, which will cause a lot of trouble to the experimenters. At present, the length of the PGK1 promoter in iGEM component library is 1497bp. We truncate 1100bp, 800bp, 600bp, 400bp or even 200bp in the existing sequence of PGK1 as the promoter sequence, and then use the red protein mCherry characterizes the startup strength of the PGK1 promoter of different lengths, trying to find the optimal PGK1 promoter with the same strength and shorter sequence compared the original promoter.
 <!-- Add more about the biology of this part here