Difference between revisions of "Part:BBa K4149086"
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Revision as of 12:31, 7 October 2022
The plasmid was modified by integrating the cyclopropane fatty acid (cfa )gene with pRSFDuet1 as a v
pRSFDuet1 is an E. coli protein dual expression vector, and we used this as a vector without T7 promoter in order to serve as a control group.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1492
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1492
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1492
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1492
Illegal NgoMIV site found at 1915 - 1000COMPATIBLE WITH RFC[1000]
