Difference between revisions of "Part:BBa K4156111"
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<partinfo>BBa_K4156111 short</partinfo> | <partinfo>BBa_K4156111 short</partinfo> | ||
− | + | pCaCd-mRFP is a biological brick that expresses mRFP under the control of the pCaCd promoter (BBa_K4156076). pCaCd-mRFP functions as a red fluorescent signal to characterize the performance of the pCaCd promoter. | |
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+ | <!-- Add more about the biology of this part here --> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | In this biobrick, we used the pCaCd promoter to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. in addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pCaCd promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pCaCd promoter to pH changes. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4156111 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4156111 SequenceAndFeatures</partinfo> |
Revision as of 06:53, 9 October 2022
pCadC-mRFP
pCaCd-mRFP is a biological brick that expresses mRFP under the control of the pCaCd promoter (BBa_K4156076). pCaCd-mRFP functions as a red fluorescent signal to characterize the performance of the pCaCd promoter.
Usage and Biology
In this biobrick, we used the pCaCd promoter to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. in addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pCaCd promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pCaCd promoter to pH changes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 731
Illegal AgeI site found at 843 - 1000COMPATIBLE WITH RFC[1000]