Difference between revisions of "Part:BBa K4156099"

 
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<partinfo>BBa_K4156099 short</partinfo>
 
<partinfo>BBa_K4156099 short</partinfo>
  
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To be refined
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pCadC-TP901 is constructed with pH-sensitive promoter pCadC and serine integrase TP901.
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<!-- Add more about the biology of this part here -->
  
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===Usage and Biology===
 
===Usage and Biology===
  
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pCadC-TP901 consists of a fusion of the pH-sensitive promoter pCadC and the serine integrase TP901. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.
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===Characterization===
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==In vitro characterization and data analysis of the reported strains==
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To improve signaling stability as well as accuracy, we added Amplifying genetic switches based on serine integrase (Bxb1,TP901) to the R reporter(BBa_) to construct the AR reporter. Fig 1 indicates pH (pCadc) induced AR reporters with homogenized fluorescence intensity (mRFP/Cell). In contrast to Fig 1 and 2, the fluorescence intensity of the AR reporter appeared more stable over time at pH 7.3 and was higher than that of the R reporter at pH 5.8, 6.3, and 7.3. This result indicates that the addition of amplifying genetic switch enhances the reporter intensity and robustness of the lactate biosensor.
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<html>
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<figure style="text-align:center;">
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4156/wiki/part/4-1.png" alt="control">
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                <figcaption><b>Figure 1:</b>  Induction of downstream gene mRFP expression over time by the AR reporter consisting of pCadC+Switch+mRFP at different pH values.</figcaption>
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              </figure>
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</html>
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<html>
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<figure style="text-align:center;">
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                <img style="max-width:700px;" src="https://static.igem.wiki/teams/4156/wiki/part/1-2-4-2.png" alt="control">
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                <figcaption><b>Figure 2:</b> Induction of downstream gene mRFP expression over time by the AR reporter consisting of pCadC +mRFP at different pH values.</figcaption>
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              </figure>
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</html>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156099 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156099 SequenceAndFeatures</partinfo>

Revision as of 14:15, 10 October 2022


pCadC-TP901

pCadC-TP901 is constructed with pH-sensitive promoter pCadC and serine integrase TP901.


Usage and Biology

pCadC-TP901 consists of a fusion of the pH-sensitive promoter pCadC and the serine integrase TP901. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.

Characterization

In vitro characterization and data analysis of the reported strains

To improve signaling stability as well as accuracy, we added Amplifying genetic switches based on serine integrase (Bxb1,TP901) to the R reporter(BBa_) to construct the AR reporter. Fig 1 indicates pH (pCadc) induced AR reporters with homogenized fluorescence intensity (mRFP/Cell). In contrast to Fig 1 and 2, the fluorescence intensity of the AR reporter appeared more stable over time at pH 7.3 and was higher than that of the R reporter at pH 5.8, 6.3, and 7.3. This result indicates that the addition of amplifying genetic switch enhances the reporter intensity and robustness of the lactate biosensor.

control
Figure 1: Induction of downstream gene mRFP expression over time by the AR reporter consisting of pCadC+Switch+mRFP at different pH values.

control
Figure 2: Induction of downstream gene mRFP expression over time by the AR reporter consisting of pCadC +mRFP at different pH values.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1632
  • 1000
    COMPATIBLE WITH RFC[1000]