Difference between revisions of "Part:BBa K4298012:Design"
GrizzlyZhao (Talk | contribs) |
GrizzlyZhao (Talk | contribs) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | pUC19 backbone, using Gibson assembly insert the EPI with α-secretion signal. | |
===Source=== | ===Source=== | ||
| − | + | Assembly with K4298009,K319003,K3105674,K4298005,K731721 and K4298011. | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
Revision as of 08:32, 7 October 2022
pUC19-ITR-EPI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1182
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1000
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 505
Design Notes
pUC19 backbone, using Gibson assembly insert the EPI with α-secretion signal.
Source
Assembly with K4298009,K319003,K3105674,K4298005,K731721 and K4298011.