Difference between revisions of "Part:BBa K4323002"

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<partinfo>BBa_K4323002 short</partinfo>
 
<partinfo>BBa_K4323002 short</partinfo>
  
This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to [https://parts.igem.org/Part:BBa_K2683006 BBa_K2683006].
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This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to [https://parts.igem.org/Part:BBa_K2683006 BBa_K2683006].  The composite part [https://parts.igem.org/Part:BBa_K4323003 BBa_K4323003] includes the necessary components to express truncated FitD toxin.
  
 
[[File:FitD toxicity.png|800px|center|FitD toxicity]]
 
[[File:FitD toxicity.png|800px|center|FitD toxicity]]

Revision as of 20:56, 11 October 2022


Truncated FitD

This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006. The composite part BBa_K4323003 includes the necessary components to express truncated FitD toxin.

FitD toxicity

Figure 1. The toxicity of Truncated FitD to Dreissena bugensis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 909
    Illegal BamHI site found at 1019
    Illegal BamHI site found at 2568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]