Difference between revisions of "Part:BBa K4323002"

Revision as of 20:56, 11 October 2022

Truncated FitD

This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006. The composite part BBa_K4323003 includes the necessary components to express truncated FitD toxin.

Figure 1. The toxicity of Truncated FitD to Dreissena bugensis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 909
Illegal BamHI site found at 1019
Illegal BamHI site found at 2568
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2191
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4323002 short</partinfo>
-This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to [https://parts.igem.org/Part:BBa_K2683006 BBa_K2683006].
+This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to [https://parts.igem.org/Part:BBa_K2683006 BBa_K2683006].  The composite part [https://parts.igem.org/Part:BBa_K4323003 BBa_K4323003] includes the necessary components to express truncated FitD toxin.
 [[File:FitD toxicity.png|800px|center|FitD toxicity]]