Difference between revisions of "Part:BBa K4268001:Experience"
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[[File: T-suny-oneonta-t7-capsid assembly protein colony PCR figure.png|200px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.]]
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[[File: T-suny-oneonta-t7-capsid assembly protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.]]
  
 
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
 
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.

Revision as of 03:22, 7 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.


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UNIQe440960d47614c6f-partinfo-00000000-QINU UNIQe440960d47614c6f-partinfo-00000001-QINU