Difference between revisions of "Part:BBa K274110"
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Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone). | Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone). | ||
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| + | Datasheet for Part BBa_K274100 and Part BBa_274110 in ''E. coli'' strain MG1655. You may also wish to refer to the "Experience" page. | ||
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| + | [[Image:Lycopene datasheet.JPG|200px]] | ||
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| + | For PDF version of this Datasheet: [[Image:Lycopene.pdf]] | ||
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Revision as of 02:29, 25 September 2009
CrtEBI under constitutive promoter
This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011.
Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene).
Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
Datasheet for Part BBa_K274100 and Part BBa_274110 in E. coli strain MG1655. You may also wish to refer to the "Experience" page.
For PDF version of this Datasheet: File:Lycopene.pdf
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2037
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1573
Illegal NgoMIV site found at 1703
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]