Difference between revisions of "Part:BBa J70500:Design"
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These primers were designed with Austin's GBW tool. | These primers were designed with Austin's GBW tool. | ||
| − | None of the annotated proteins have CGG codons. | + | None of the annotated proteins have CGG codons. OrfB (rep) has a UGA stop codon, which will be read through to the next stop in M. florum. |
There are no GATC sites. | There are no GATC sites. | ||
Revision as of 17:36, 18 September 2009
pWV01 broad host range plasmid origin and rep protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Avoided a single Sau3AI site near the claI site, to eliminate Sau3AI sites in the final plasmid assembly.
- PCR with primers:
- WV-F: gtttcttcgaattcgcggccgcttctagagcgattttttattaaaacgtctcaaaatcgtttctg
- WV-R: gtttcttcctgcagcggccgctactagtaatcattttgtttattgcaattgtattgctattaatcg
These primers were designed with Austin's GBW tool.
None of the annotated proteins have CGG codons. OrfB (rep) has a UGA stop codon, which will be read through to the next stop in M. florum.
There are no GATC sites.
Source
PCR of the plasmid pMG, kind gift from Prof. Jan Kok. This plasmid contains the ClaI fragment of the pWV01 plasmid.