Difference between revisions of "Part:BBa K4417000"
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<h1>Usage and Biology</h1> | <h1>Usage and Biology</h1> | ||
| − | This | + | This mutated plasmid can be used as a shuttle vector for both ''B.subtilis'' and ''E.coli''. |
<h1>References</h1> | <h1>References</h1> | ||
Revision as of 08:03, 7 October 2022
pCT5c Type IIS Compatible Plasmid
Description
Shuttle vecor for Bacillus subtilis. Mutated pCT5-bac2.0 by removing three BsaI sites to make it TypeIIS compatible.
Usage and Biology
This mutated plasmid can be used as a shuttle vector for both B.subtilis and E.coli.
References
1. Development of a synthetic cumate-inducible gene expression system for Bacillus. Seo SO, Schmidt-Dannert C. Appl Microbiol Biotechnol. 2018 Nov 3. pii: 10.1007/s00253-018-9485-4. doi: 10.1007/s00253-018-9485-4. 10.1007/s00253-018-9485-4 PubMed 30392122
2. Addgene plasmid # 119872; http://n2t.net/addgene:119872; RRID:Addgene_119872
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3926
Illegal EcoRI site found at 6982
Illegal XbaI site found at 7437
Illegal SpeI site found at 1
Illegal PstI site found at 3174 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3926
Illegal EcoRI site found at 6982
Illegal NheI site found at 7213
Illegal SpeI site found at 1
Illegal PstI site found at 3174 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3926
Illegal EcoRI site found at 6982
Illegal BamHI site found at 907 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3926
Illegal EcoRI site found at 6982
Illegal XbaI site found at 7437
Illegal SpeI site found at 1
Illegal PstI site found at 3174 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3926
Illegal EcoRI site found at 6982
Illegal XbaI site found at 7437
Illegal SpeI site found at 1
Illegal PstI site found at 3174
Illegal NgoMIV site found at 1937 - 1000COMPATIBLE WITH RFC[1000]