Difference between revisions of "Part:BBa K4165120"

Revision as of 13:24, 10 October 2022

HTRA Binding Peptide 26

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC50 of 0.26 ± 0.01 and ΔG of 13 kJ mol-1. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 20.83, molprobity = 3.85, ramachandran_favored = 33.33, ramachandran_outliers = 33.33, Qmean_4 = -6.45183, Qmean6 = -5.51397

Modeling

                 Figure 1.: 3D Model of H1Z peptide Modelled by AlphaFold2.0 displayed in Pyomol

References

Beuming, T., Farid, R., & Sherman, W. (2009). High‐energy water sites determine peptide binding affinity and specificity of PDZ domains. Protein Science, 18(8), 1609-1619.

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC50 of 0.26 ± 0.01 and G of 13
+Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC50 of 0.26 ± 0.01 and ΔG of 13 kJ mol-1.
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 <span class='h3bb'>Sequence and Features</span>