Difference between revisions of "Part:BBa K4368001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Consideration should be given to the correct optimization of the sequence for use in different organisms. | |
Revision as of 22:52, 6 October 2022
pcstA + rbs + yebF + cex + terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1044
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 1050
Illegal NgoMIV site found at 1552 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1097
Illegal SapI.rc site found at 1180
Design Notes
Consideration should be given to the correct optimization of the sequence for use in different organisms.
Source
The cex gene is from Cellulomonas fimi, while the remaining parts are from Escherichia coli. The whole construct has been optimised for use in E. coli.