Difference between revisions of "Part:BBa K228004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression. | |
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===Source=== | ===Source=== | ||
Latest revision as of 04:18, 13 October 2009
NahR( reverse) - salicylate promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.
Source
It is constrcuted by PCR from the plasmid kindly provided by Voigt Lab in UCSF.