Difference between revisions of "Part:BBa K274120:Experience"

 
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===User Reviews===
 
===User Reviews===
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Characterization by UiOslo_Norway 2019
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This part was of special interest to our team. The part consists of homologs of the genes we used; crtE, crtB and crtI from Deinococcus radiodurans. BBa_K274120 has individual ribosomal binding sites for each individual enzyme. The part that we made had a single ribosomal binding site followed by all three genes in a single open reading frame. The first two genes had their stopcodons removed (see BBa_K2971010).
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<img style="width:40% !important;" src="https://2019.igem.org/wiki/images/9/99/T--UiOslo_Norway--HQ%26pBad.jpg?">
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<strong>Figure 1: Induction of BBa_K274120 in Escherichia coli (BL21)</strong></br>
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Expression was induced in 2&#x25; arabinose </br>
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From left to right &#58;Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, uinduced E.coli with BBa_K274120, induced E.coli with BBa_K274120.
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<img style="width:40% !important;" src="https://2019.igem.org/wiki/images/4/4e/T--UiOslo_Norway--our%26pBad.jpg">
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<strong>Figure 2: Expression of crtEBI in Escherichia coli (BL21)</strong></br>
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Expression was induced in 2&#x25; arabinose </br>
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From left to right &#58;Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, induced E.coli with crtEBI expression vector, uninduced E.coli with crtEBI expression vector.
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Revision as of 16:21, 21 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K274120

User Reviews

Characterization by UiOslo_Norway 2019

This part was of special interest to our team. The part consists of homologs of the genes we used; crtE, crtB and crtI from Deinococcus radiodurans. BBa_K274120 has individual ribosomal binding sites for each individual enzyme. The part that we made had a single ribosomal binding site followed by all three genes in a single open reading frame. The first two genes had their stopcodons removed (see BBa_K2971010).

Figure 1: Induction of BBa_K274120 in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, uinduced E.coli with BBa_K274120, induced E.coli with BBa_K274120.

Figure 2: Expression of crtEBI in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, induced E.coli with crtEBI expression vector, uninduced E.coli with crtEBI expression vector.


UNIQ8e8431b0de0ee528-partinfo-00000001-QINU UNIQ8e8431b0de0ee528-partinfo-00000002-QINU