Difference between revisions of "Part:BBa K4385020"
Line 3: | Line 3: | ||
<partinfo>BBa_K4385020 short</partinfo> | <partinfo>BBa_K4385020 short</partinfo> | ||
− | a | + | This part is composed of ZraSR two-component signal transport system, PzraP and OmpC-ZBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PzraP promoter promotes the transcription of downstream OmpC-ZBP, and realizes the adsorption of zinc ions in the environment. Add 6*Hig Tag to this sequence to purify recombinant protein. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 09:41, 7 October 2022
Zinc-inducible surface display
This part is composed of ZraSR two-component signal transport system, PzraP and OmpC-ZBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PzraP promoter promotes the transcription of downstream OmpC-ZBP, and realizes the adsorption of zinc ions in the environment. Add 6*Hig Tag to this sequence to purify recombinant protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 616
Illegal SpeI site found at 775
Illegal PstI site found at 1123 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 616
Illegal NheI site found at 1506
Illegal NheI site found at 1529
Illegal SpeI site found at 775
Illegal PstI site found at 1123 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 616
Illegal BglII site found at 324
Illegal BglII site found at 1641 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 616
Illegal SpeI site found at 775
Illegal PstI site found at 1123 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 616
Illegal SpeI site found at 775
Illegal PstI site found at 1123
Illegal AgeI site found at 442 - 1000COMPATIBLE WITH RFC[1000]