Difference between revisions of "Part:BBa K4385019"
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<partinfo>BBa_K4385019 short</partinfo> | <partinfo>BBa_K4385019 short</partinfo> | ||
− | .. | + | This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of zinc ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein. |
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Revision as of 09:39, 7 October 2022
Copper-inducible surface display
This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of zinc ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 535
Illegal XbaI site found at 28
Illegal SpeI site found at 694
Illegal PstI site found at 1042 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 535
Illegal NheI site found at 1410
Illegal NheI site found at 1433
Illegal SpeI site found at 694
Illegal PstI site found at 1042 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 535
Illegal BglII site found at 243
Illegal BglII site found at 1545 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 535
Illegal XbaI site found at 28
Illegal SpeI site found at 694
Illegal PstI site found at 1042 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 535
Illegal XbaI site found at 28
Illegal SpeI site found at 694
Illegal PstI site found at 1042
Illegal AgeI site found at 361 - 1000COMPATIBLE WITH RFC[1000]