Difference between revisions of "Part:BBa K4245001:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
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The Spinach aptamer was originally generated by the Jaffrey Lab at Cornell University using the SELEX (systematic evolution of ligands by exponential enrichment) process (add reference).
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The part was acquired as synthesized sequences from IDT and Twist Bioscience.
  
 
===References===
 
===References===
 
Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c
 
Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c

Revision as of 14:09, 6 October 2022


Spinach aptamer with LacI repression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 361
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Spinach aptamer was originally generated by the Jaffrey Lab at Cornell University using the SELEX (systematic evolution of ligands by exponential enrichment) process (add reference).

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c