Difference between revisions of "Part:BBa K4245001:Design"
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===Source=== | ===Source=== | ||
| − | + | The Spinach aptamer was originally generated by the Jaffrey Lab at Cornell University using the SELEX (systematic evolution of ligands by exponential enrichment) process (add reference). | |
===References=== | ===References=== | ||
Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c | Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c | ||
Revision as of 14:05, 6 October 2022
Spinach aptamer with LacI repression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 361
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The composite part was acquired from DNA synthesis companies. The part was transformed into standard iGEM high copy plasmids.
Source
The Spinach aptamer was originally generated by the Jaffrey Lab at Cornell University using the SELEX (systematic evolution of ligands by exponential enrichment) process (add reference).
References
Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c