Difference between revisions of "Part:BBa K4165142"
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MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils. | MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils. | ||
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===Dry Lab=== | ===Dry Lab=== | ||
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===References=== | ===References=== |
Revision as of 17:01, 10 October 2022
MMD3rev Peptide
Tau binding peptide targeting the PHF seed of Tau
Usage and Biology
MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry Lab
Modeling
MMD3rev is modeled by AlphaFold2, Apptest, ITASSER and TrRosetta, best model obtained from ITASSER.
Figure 1.: Predicted 3D structure of Synthetic peptide MMD3rev.
Table 1: Quality assessment parameters of MMD3rev model.
References
1. Malhis, M., Kaniyappan, S., Aillaud, I., Chandupatla, R. R., Ramirez, L. M., Zweckstetter, M., ... & Funke, S. A. (2021). Potent Tau Aggregation Inhibitor D‐Peptides Selected against Tau‐Repeat 2 Using Mirror Image Phage Display. ChemBioChem, 22(21), 3049-3059.