Difference between revisions of "Part:BBa K4245001:Design"

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===Source===
 
===Source===
  
−
The part was acquired as synthesized sequences from IDT and Twist Bioscience.
+
Centre for Synthetic Biology and Innovation, Imperial College London
  
 
===References===
 
===References===
 +
Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c

Revision as of 13:59, 6 October 2022


Spinach aptamer with LacI repression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 361
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part was acquired from DNA synthesis companies. The part was transformed into standard iGEM high copy plasmids.

Source

Centre for Synthetic Biology and Innovation, Imperial College London

References

Pothoulakis, G., Ceroni, F., Reeve, B., & Ellis, T. (2013). The spinach RNA aptamer as a characterization tool for synthetic biology. ACS Synthetic Biology, 3(3), 182–187. https://doi.org/10.1021/sb400089c