Difference between revisions of "Part:BBa K4361000"
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+ | Firstly, the wildtype sequence was scrambled using the Genscript Sequence Scramble tool (https://www.genscript.com/tools/create-scrambled-sequence) to create an oligo containing the same nucleotides in a randomized order. Since this oligo does not contain either of the inverted repeat pairs, BlcR should not be able to specifically bind this molecule, thus it can be used as a negative control to compare other sequences to. This oligo was designated BBa_K4361000. | ||
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Shares nucleotides of BBa_K4361001, but in a scrambled order as to no longer allow specific binding. | Shares nucleotides of BBa_K4361001, but in a scrambled order as to no longer allow specific binding. | ||
− | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:51, 6 October 2022
BlcR-binding oligo, 51 bp, scrambled
Firstly, the wildtype sequence was scrambled using the Genscript Sequence Scramble tool (https://www.genscript.com/tools/create-scrambled-sequence) to create an oligo containing the same nucleotides in a randomized order. Since this oligo does not contain either of the inverted repeat pairs, BlcR should not be able to specifically bind this molecule, thus it can be used as a negative control to compare other sequences to. This oligo was designated BBa_K4361000.
Shares nucleotides of BBa_K4361001, but in a scrambled order as to no longer allow specific binding.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 37
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 37
- 1000COMPATIBLE WITH RFC[1000]