Difference between revisions of "Part:BBa K4162029"

 
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<partinfo>BBa_K4162029 short</partinfo>
 
<partinfo>BBa_K4162029 short</partinfo>
  
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Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.
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===Introduction===
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[[File:T--Fudan--logo-Rester-rectangle.jpg|100px|right|2021 Fudan]]
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Coding sequence of BCMO and gene ''ybbO'' separated by hammerhead ribozyme. Gene ''ybbO'' is tagged with tdMCP and EGFP.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 07:51, 7 October 2022


ribozyme + T7_RBS + BCMO + ribozyme + T7_RBS + ybbO-tdMCP-EGFP

Introduction

2021 Fudan

Coding sequence of BCMO and gene ybbO separated by hammerhead ribozyme. Gene ybbO is tagged with tdMCP and EGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2521
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2521
    Illegal AgeI site found at 1018
    Illegal AgeI site found at 1611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2101
    Illegal BsaI site found at 2455