Difference between revisions of "Part:BBa K4368001"

Revision as of 15:13, 6 October 2022

pcstA + rbs + yebF + cex + terminator

Description

Cex encodes for the exoglucanase gene of Cellulomonas fimi (BBa_K118022). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cenA and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (BBa_K1610300) that secrete the enzyme out of the E. coli membrane. The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Characterization

xxx

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1044
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 1050
Illegal NgoMIV site found at 1552
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1097
Illegal SapI.rc site found at 1180

Contribution

Group: [1]
Author: Molina Calvo, Alonso

@@ Line 4: / Line 4: @@
 ==Description==
-''Cex'' encodes for the exoglucanase gene of ''Celullomonas fimi'' (<partinfo>BBa_K118022</partinfo>). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cenA and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by glucose concentration (<partinfo>BBa_K118011</partinfo>). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (<partinfo>BBa_K1610300</partinfo>) that secrete the enzyme out of the ''E. coli'' membrane.
+''Cex'' encodes for the exoglucanase gene of ''Cellulomonas fimi'' (<partinfo>BBa_K118022</partinfo>). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cenA and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by glucose concentration (<partinfo>BBa_K118011</partinfo>). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (<partinfo>BBa_K1610300</partinfo>) that secrete the enzyme out of the ''E. coli'' membrane.
 The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.