Difference between revisions of "Part:BBa K4368000"

Revision as of 15:12, 6 October 2022

pcstA + rbs + yebF + cenA + terminator

Description

CenA encodes for the endoglucanase gene of Cellulomonas fimi (BBa_K118023). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (BBa_K1610300) that secrete the enzyme out of the E. coli membrane. The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Characterization

xxx

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 554
Illegal NotI site found at 1698
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 268
Illegal BglII site found at 1611
Illegal BamHI site found at 747
Illegal XhoI site found at 1109
Illegal XhoI site found at 1358
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 889
Illegal NgoMIV site found at 1814
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 793

Contribution

Group: [1]
Author: Molina Calvo, Alonso

@@ Line 3: / Line 3: @@
 ==Description==
-''CenA'' encodes for the endoglucanase gene of ''Celullomonas fimi'' (<partinfo>BBa_K118023</partinfo>). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by glucose concentration (<partinfo>BBA_K118011</partinfo>). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (<partinfo>BBa_K1610300</partinfo>) that secrete the enzyme out of the ''E. coli'' membrane.
+''CenA'' encodes for the endoglucanase gene of ''Cellulomonas fimi'' (<partinfo>BBa_K118023</partinfo>). This enzyme is responsible of the degradation of cellulose working coordinated with th genes cex and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (<partinfo>BBa_B0030</partinfo>), a double terminator (<partinfo>BBa_B0015</partinfo>) as well as a promoter inducible by glucose concentration (<partinfo>BBA_K118011</partinfo>). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (<partinfo>BBa_K1610300</partinfo>) that secrete the enzyme out of the ''E. coli'' membrane.
 The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.