Difference between revisions of "Part:BBa K4413993"

 
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NOTOC__
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<partinfo>BBa_K4413993 short</partinfo>
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gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4413993 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4413993 parameters</partinfo>
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Revision as of 07:12, 8 October 2022

NOTOC__ PGroES-RiboJ-DrRBS-gabY

gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 476
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 390
    Illegal NgoMIV site found at 482
    Illegal NgoMIV site found at 648
  • 1000
    COMPATIBLE WITH RFC[1000]