Difference between revisions of "Part:BBa K4387978"

Revision as of 17:52, 5 October 2022

Nitric Oxide Sensing Genetic Circuit

This composite part contains the nitric oxide sensor pNorVß, followed by 2 different ribosome binding sites. The promoter induces upon NO binding the expression of a monovalent anti-TNF nanobodoy BBa_K4387996. The additional NorR at the end of the composite part enhances the postitive feedback-loop, increasing the response of the promoter to NO. Together with the hemolysin A secretion machinery BBa_K4387987, a complete genetic circuit is obtained that allows secretion of nanobodies or if exchanged, secretion of other proteins of interest.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 708
Illegal BglII site found at 1287
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 17:51, 5 October 2022 (view source) Nathi (Talk \| contribs) ← Older edit		Revision as of 17:52, 5 October 2022 (view source) Nathi (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4387978 short</partinfo>		<partinfo>BBa_K4387978 short</partinfo>

−	This composite part contains the nitric oxide sensor pNorVß, followed by 2 different ribosome binding sites. The promoter induces upon NO binding the expression of a monovalent anti-TNF nanobodoy (BBa_K4387996). The additional NorR at the end of the composite part enhances the postitive feedback-loop, increasing the response of the promoter to NO. Together with the hemolysin A secretion machinery <partinfo>BBa_K4387987</partinfo>, a complete genetic circuit is obtained that allows secretion of nanobodies or if exchanged, secretion of other proteins of interest.	+	This composite part contains the nitric oxide sensor pNorVß, followed by 2 different ribosome binding sites. The promoter induces upon NO binding the expression of a monovalent anti-TNF nanobodoy <partinfo>BBa_K4387996</partinfo>. The additional NorR at the end of the composite part enhances the postitive feedback-loop, increasing the response of the promoter to NO. Together with the hemolysin A secretion machinery <partinfo>BBa_K4387987</partinfo>, a complete genetic circuit is obtained that allows secretion of nanobodies or if exchanged, secretion of other proteins of interest.