Difference between revisions of "Part:BBa K4321001"
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This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP). | This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells. | This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells. | ||
Revision as of 17:46, 5 October 2022
BFP
This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP).
Usage and Biology
This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]