Difference between revisions of "Part:BBa K4204007"
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− | ==Quality control system + LysPBC5== | + | ==Quality control system + LysPBC5 Ori== |
===Description=== | ===Description=== |
Latest revision as of 17:38, 5 October 2022
Quality control system + LysPBC5 Ori
Description
The quality control system is added to LysPBC5. Protein quality control systems include a switch at the 5’ end of an mRNA and a corresponding trigger sequence with reverse complementary to the switch at the 3’ end of the mRNA. When the Cis-trigger appears, the switch will bind with the trigger preferentially because the Cis-trigger has a higher affinity with the switch. Or, the switch binds with its complementary sequence and clocks the RBS inside a neck-ring structure; this blocks the transcription initiation. The QC system applied to the LysPBC5 increase the amount of functional protein. Other IGEM teams can use the tested ProQC system for full-length protein expression to increase the amount of functional protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 706
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 758
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1004