Difference between revisions of "Part:BBa J37032:Experience"

Revision as of 11:02, 10 September 2009

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Applications of BBa_J37032

User Reviews

UNIQ3c5134a5c004359d-partinfo-00000000-QINU

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Aberdeen_Scotland 2009

This part was used to test our LuxR/LuxI operon (K182200). For that purpose J37032 has been transformed in SCS1 E.coli cells as negative control and furthermore SCS1 cells have been transformed with J37032 and K182200 (called pIR in below figure). A second control was the transformance of SCS1 cells with K182200 only. It was found that this testing construct produces the same amount of GFP with or without the presence of LuxR. J37032 is hence not responsive to LuxR and cannot be used to test quorum sensing. The Figure below shows the negative controls (J37032 alone and pIR alone) as well as both biobricks together under a light and fluorescence microscope.

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 <I> Aberdeen_Scotland 2009 </I>
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-Aberdeen info
+This part was used to test our LuxR/LuxI operon (K182200). For that purpose J37032 has been transformed in SCS1 E.coli cells as negative control and furthermore SCS1 cells have been transformed with J37032 and K182200 (called pIR in below figure). A second control was the transformance of SCS1 cells with K182200 only. It was found that this testing construct produces the same amount of GFP with or without the presence of LuxR. J37032 is hence not responsive to LuxR and cannot be used to test quorum sensing. The Figure below shows the negative controls (J37032 alone and pIR alone) as well as both biobricks together under a light and fluorescence microscope.
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 [[Image:J37TEST_figure.jpg|center|400 px]]