Difference between revisions of "Part:BBa K4207002"

Revision as of 12:13, 5 October 2022

BYDV toehold switch A70

Toehold switch for the detection of BYDV gRNA

1. Usage and Biology

Toehold switches are de novo designed riboregulators that can be used to sense different nucleic acid sequences. They are specifically designed RNA sequences that have the ribosome binding site (RBS) and the start codon in a stem-loop followed by a reporter gene. The RBS and the start codon are sequestered in the secondary structure, which hinder the translation of the reporter gene. The toehold switch has a specific binding site to its trigger sequence, which extends to the base of the stem-loop. When the trigger binds, it unwinds the lower part of the stem-loop, leaving only a weak secondary structure intact. This remaining structure is designed to be weak, so ribosome binding unwinds the structure, allowing translation to occur. (Green et. al., 2014) (Green et. al., 2017).

Here we designed a A-series toehold switch, which has a structure allowing lower translational leakage to previous toehold switches (Pardee et. al., 2016). This toehold switch is designed to detect the presence of barley yellow dwarf virus gRNA by binding to a conserved sequence found in the virus' enome.

Sequence and Features

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2. Design

3. Characterization

4. Conclusion

5. References