Difference between revisions of "Part:BBa K4137001"
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BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of | BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of | ||
− | a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and | + | a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinction from CcdA. |
+ | |||
+ | [[File:ccdb-myc.png|800px|thumb|center|Fig.1 ccdB with a C-Terminal Myc tag.]] | ||
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Revision as of 03:38, 9 October 2022
CcdB-Myc Toxin
BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinction from CcdA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 216