Difference between revisions of "Part:BBa K4121023:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.
+
Before using this part, please check to whether the operated sequences contain any enzyme site required for the construction. If so, please consider to change it using site-directed mutagenesis.
−
 
+
  
  

Latest revision as of 11:31, 7 October 2022


The mevalonate phosphokinase gene ERG8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 916
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 916
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 916
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before using this part, please check to whether the operated sequences contain any enzyme site required for the construction. If so, please consider to change it using site-directed mutagenesis.


Source

National Center for Biotechnology Information (NCBI)

References