Difference between revisions of "Part:BBa K4167000"
| Line 21: | Line 21: | ||
[[File:K4167000-fig.1.jpg|center]] | [[File:K4167000-fig.1.jpg|center]] | ||
<br/> | <br/> | ||
| + | Fig.1 The map of toehold switch-amilCP described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites). | ||
<br/> | <br/> | ||
| + | <br/> | ||
| + | After detecting the restriction enzyme information of toehold switch-amilCP using SnapGene software, it was inserted into the pSB1C3 plasmid to construct the standard part pSB1C3-toehold switch-amilCP with PCR method. Then it was identified as follows: | ||
| + | <br/> | ||
| + | <br/> | ||
| + | [[File:K4167000-fig.2.jpg|center]] | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 04:07, 5 October 2022
toehold switch-amilCP
Toehold switch-amilCP was inserted into pSB1C3 which is designed to express amilCP protein triggered by miRNA 34a-5p. It is used to detect the amount of miRNA 34a-5p in samples.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows:
Fig.1 The map of toehold switch-amilCP described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites).
After detecting the restriction enzyme information of toehold switch-amilCP using SnapGene software, it was inserted into the pSB1C3 plasmid to construct the standard part pSB1C3-toehold switch-amilCP with PCR method. Then it was identified as follows:
