Difference between revisions of "Part:BBa K4385015"
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<partinfo>BBa_K4385015 short</partinfo> | <partinfo>BBa_K4385015 short</partinfo> | ||
− | + | This part was mainly composed of constitutive promoter J23100, the anchoring protein OmpC-CBP and the heavy metal detoxification protein. Promoter J23100 continuously expressed downstream copper ion surface display proteins to verify the function of outer membrane protein C and lipoprotein of copper ion surface display system. The 6*His Tags were added to this sequence to purify the recombinant protein. | |
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Revision as of 16:12, 6 October 2022
Copper constitutive surface display
This part was mainly composed of constitutive promoter J23100, the anchoring protein OmpC-CBP and the heavy metal detoxification protein. Promoter J23100 continuously expressed downstream copper ion surface display proteins to verify the function of outer membrane protein C and lipoprotein of copper ion surface display system. The 6*His Tags were added to this sequence to purify the recombinant protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 414
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1289
Illegal NheI site found at 1312
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 414
Illegal BglII site found at 122
Illegal BglII site found at 1424 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921
Illegal AgeI site found at 240 - 1000COMPATIBLE WITH RFC[1000]