Difference between revisions of "Part:BBa K4204003"
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This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage.
 
This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4204003 SequenceAndFeatures</partinfo>

Revision as of 00:43, 12 October 2022

Description

This sequence contains 3 protection bases at the 5’ end of the sequence, which is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-HBLA region pairs with the targeted B.cereus HBLA gene sequence and enables cleavage.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 26
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]