Difference between revisions of "Part:BBa K4268000:Experience"

Revision as of 22:16, 4 October 2022

The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.

Applications of BBa_K4268000

User Reviews

UNIQ4ada5dc4c24c400a-partinfo-00000000-QINU UNIQ4ada5dc4c24c400a-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha cells.  After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
-how you used this part and how it worked out.
+[[File: T-suny-oneonta-t7-head-tail connector colony PCR figure.jpg|200px|border|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.]]
+The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
 ===Applications of BBa_K4268000===