Difference between revisions of "Part:BBa K4368004"

Revision as of 17:33, 4 October 2022

pcI + rbs + glgC16 + terminator

GlgC16 encodes for the ADP-glucose pyrophosphorylase gene of Escherichia coli (BBa_K118016). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs BBa_B0030, a double terminator (BBa_B0015) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 272
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4368004 short</partinfo>
-''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' (bbpart>BBa_K118016</bbpart>). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs <bbpart>BBa_B0030</bbpart>, a double terminator (<bbpart>BBa_B0015</bbpart>) as well as a promoter inducible by the cI protein of the ''phage lambda'' (<bbpart>BBa_R1051</bbpart>).
+''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' (<bbpart>BBa_K118016</bbpart>). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs <bbpart>BBa_B0030</bbpart>, a double terminator (<bbpart>BBa_B0015</bbpart>) as well as a promoter inducible by the cI protein of the ''phage lambda'' (<bbpart>BBa_R1051</bbpart>).
 The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.