Difference between revisions of "Part:BBa K4368004"
Line 5: Line 5:
 
''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K118016 BBa_K118016]). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]), a double terminator ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) as well as a promoter inducible by the cI protein of the phage lambda ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R1051 BBa_R1051]).
 
''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K118016 BBa_K118016]). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]), a double terminator ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) as well as a promoter inducible by the cI protein of the phage lambda ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R1051 BBa_R1051]).
 
The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
 
The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
 +
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K4368007 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K4368007 parameters</partinfo>
 +
<!-- -->

Revision as of 16:47, 4 October 2022


pcI + rbs + glgC16 + terminator

GlgC16 encodes for the ADP-glucose pyrophosphorylase gene of Escherichia coli (BBa_K118016). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 633
    Illegal AgeI site found at 745
  • 1000
    COMPATIBLE WITH RFC[1000]