Difference between revisions of "Part:BBa K4140017"
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==Usage== | ==Usage== | ||
| − | We used a mammalian expression systems to regulate the expression of Cas12g which have a remarkable role on tuning and controlling our circuit activity and the levels of PAH to be constant within normal level to avoid | + | We used a mammalian expression systems to regulate the expression of Cas12g which have a remarkable role on tuning and controlling our circuit activity and the levels of PAH to be constant within normal level to avoid overexpression of cas12g |
==Literature Characterization== | ==Literature Characterization== | ||
Revision as of 11:11, 4 October 2022
CMV promoter
Part Description
CMV (cytomegalovirus) promoter is a strong constitutive promoter commonly used in mammalian expression systems to produce high levels of expression, However CMV promoter is on of the most variable types of promoter as it is highly variable between different cell types such as being strong in some cell types and being much weaker in other types
Usage
We used a mammalian expression systems to regulate the expression of Cas12g which have a remarkable role on tuning and controlling our circuit activity and the levels of PAH to be constant within normal level to avoid overexpression of cas12g
Literature Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]