Difference between revisions of "Part:BBa K4212039:Design"
 
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===Design Notes===
 
===Design Notes===
  
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We intended to construct a Level 1 Golden Gate Plasmid by combining the native promoter of CotG, the CotG-chitinase chimera. We aimed to compared the transcription effectiveness of the native promoter with the hyperspank promoter, and we would select the more suitable one for future Golden Gate assembly. We would then change our construct design and the media we use accordingly. tL3S2P21 is a strong promoter, which helps to increase the production of desired protein.
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We intended to construct a Level 1 Golden Gate Plasmid by combining the native promoter of CotG, the CotG-chitinase fusion protein. We aimed to compared the transcription effectiveness of the native promoter with the hyperspank promoter, and we would select the more suitable one for future Golden Gate assembly. We would then change our construct design and the media we use accordingly. tL3S2P21 is a strong terminator, which helps to increase the production of desired protein.
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===Source===
 
===Source===

Latest revision as of 11:19, 9 October 2022


ChiS6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1062
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1062
    Illegal NheI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1062
    Illegal BglII site found at 101
    Illegal BamHI site found at 1047
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1062
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1062
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We intended to construct a Level 1 Golden Gate Plasmid by combining the native promoter of CotG, the CotG-chitinase fusion protein. We aimed to compared the transcription effectiveness of the native promoter with the hyperspank promoter, and we would select the more suitable one for future Golden Gate assembly. We would then change our construct design and the media we use accordingly. tL3S2P21 is a strong terminator, which helps to increase the production of desired protein.

Source

Synthetic construct

References

https://parts.igem.org/Part:BBa_K143015