Revision as of 09:26, 4 October 2022

J23100-RFP

BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.

The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 616
Illegal AgeI site found at 728
1000
COMPATIBLE WITH RFC[1000]

Part Documentation by TU_Dresden 2022 Team

Table of content
Biology Expression Comparison to constitutative promoter Item4

Biology
Expression
Comparison to constitutative promoter
Item4

Part was compared to BBa_J04450.

Figure 1: Test description

@@ Line 13: / Line 13: @@
 =Part Documentation by TU_Dresden 2022 Team=
-Table of content:
+<table border=1; border-collapse=collapse;>
+<tr><th>Table of content</th></tr>
+<tr>
+<td align="left">
 # Biology
 # Expression
 # Comparison to constitutative promoter
 # Item4
+</td>
+</tr>
+</table>
-Part was compared to [https://parts.igem.org/Part:BBa_J04450 BBa_J04450.]
+# Biology
+# Expression
+# Comparison to constitutative promoter
+# Item4
+Part was compared to [https://parts.igem.org/Part:BBa_J04450 BBa_J04450.]
 [[File:test.png|thumb|center|850px|

Difference between revisions of "Part:BBa K2205000"

Revision as of 09:26, 4 October 2022

Part Documentation by TU_Dresden 2022 Team

Usage and Biology

TEst