Difference between revisions of "Part:BBa K2205000"

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=Part Documentation by TU_Dresden 2022 Team=
 
=Part Documentation by TU_Dresden 2022 Team=
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Table of content:
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# Biology
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# Expression
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# Comparison to constitutative promoter
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# Item4
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Part was compared to [https://parts.igem.org/Part:BBa_J04450 BBa_J04450.]
 
Part was compared to [https://parts.igem.org/Part:BBa_J04450 BBa_J04450.]
  

Revision as of 08:56, 4 October 2022


J23100-RFP

BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.

The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]

Part Documentation by TU_Dresden 2022 Team

Table of content:

  1. Biology
  2. Expression
  3. Comparison to constitutative promoter
  4. Item4

Part was compared to BBa_J04450.

Figure 1: Test description


Usage and Biology

TEst