Difference between revisions of "Part:BBa K4298012"

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PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends.
 
PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends.
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The original inverted repeat sequence at each end of the insertion fragment, which assists the integration of the insertion sequence into the P1 plasmid
 
  
 
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Revision as of 06:09, 7 October 2022


pUC19-ITR-EPI

PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1000
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505