Difference between revisions of "Part:BBa K4268000:Experience"

Revision as of 22:44, 3 October 2022

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Applications of BBa_K4268000

The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1:Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product using VF/VR primers is 1739 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.

User Reviews

UNIQ3fed3285f370ca88-partinfo-00000000-QINU UNIQ3fed3285f370ca88-partinfo-00000001-QINU

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-T-suny-oneonta-t7-head-tail connector colony PCR figure.png
+[[File:T-suny-oneonta-t7-head-tail connector colony PCR figure.png|200px|border|center|Figure 1:Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product using VF/VR primers is 1739 bp.]]
-Caption: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product using VF/VR primers is 1739 bp.
 The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.