Difference between revisions of "Part:BBa K4390009"
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==Characterization== | ==Characterization== | ||
| − | Mytilus edulis MT sequence was assembled into plasmid BBa_J428341 along with [[Part:BBa_K4390017|BBa_K4390017]] and BBa_K4390016. To confirm the assembly was success, we performed blue-white screening and colony PCR | + | Mytilus edulis MT sequence was assembled into plasmid BBa_J428341 along with [[Part:BBa_K4390017|BBa_K4390017]] and [[Part:BBa_K4390016|BBa_K4390016]]. To confirm the assembly was success, we performed blue-white screening and colony PCR |
[[File:ME_MT.png|centre|link=]] | [[File:ME_MT.png|centre|link=]] | ||
Revision as of 22:10, 3 October 2022
Mytilus edulis Metallothionein
Usage and Biology
Metallothionein (MT) is a small protein (around 6-7 kDa) which is rich in cysteine. These thiol group in cysteines provide ability to chelate almost all heavy metal ions including Cd2+, Hg2+, Pb2+ and As3+, but had been shown that has higher binding affinity with Hg2+ (Manceau, A. et al., 2019). The ability of chelating heavy metals provides the metal tolerance for its hosts. For its ability to binding heavy metal strongly, this part can be used to build structure which can capture heavy metal ions in aqueous environment. This MT sequence was obtained from Mytilus edulis, a blue mussel which originally live in aqueous environment (Manceau, A. et al., 2019) and designed as a C part for JUMP assembly (Valenzuela-Ortega M and French C., 2021).
Characterization
Mytilus edulis MT sequence was assembled into plasmid BBa_J428341 along with BBa_K4390017 and BBa_K4390016. To confirm the assembly was success, we performed blue-white screening and colony PCR
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]