Difference between revisions of "Part:BBa K4202047"
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To select and sequence the exogenous fragments introduced to plasmid pHY300PLK, we designed a pair of primers located upstream and downstream of the MCS on the plasmid backbone. This is the forward primer upstream of the MCS. | To select and sequence the exogenous fragments introduced to plasmid pHY300PLK, we designed a pair of primers located upstream and downstream of the MCS on the plasmid backbone. This is the forward primer upstream of the MCS. | ||
| + | <br> | ||
| + | See the reverse primer in <partinfo>BBa_K4202048</partinfo> | ||
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Revision as of 02:56, 4 October 2022
primer B.S - PHY - F
To select and sequence the exogenous fragments introduced to plasmid pHY300PLK, we designed a pair of primers located upstream and downstream of the MCS on the plasmid backbone. This is the forward primer upstream of the MCS.
See the reverse primer in BBa_K4202048
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]