Difference between revisions of "Part:BBa K4385013"

 
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<partinfo>BBa_K4385013 short</partinfo>
 
<partinfo>BBa_K4385013 short</partinfo>
  
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This part consists of the constitutive promoters J23100, OmpC (BBa_K4385003) and ZBP (BBa_K4385004), which is used to verify the function of the zinc ion surface display parts. The 6*His tag was added to this sequence to purify the recombinant protein.
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The zinc sensor part consists of the PzraP promoter, ZraSR two-component membrane associated sensor kinase system and GFP. ZraSR consists of histidine kinase ZraS, cytoplasmic transcription factor ZraR and periplasmic accessory protein ZraP, responsible for sensing the presence of external Zn2+. The ZraSR system regulates the expression of zraP, which encodes a periplasmic zinc-binding protein, in response to high concentrations of extracellular zinc ions; phosphorylated ZraR triggers divergent transcription of zraP and zraSR.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 14:31, 6 October 2022


Zinc sensor

The zinc sensor part consists of the PzraP promoter, ZraSR two-component membrane associated sensor kinase system and GFP. ZraSR consists of histidine kinase ZraS, cytoplasmic transcription factor ZraR and periplasmic accessory protein ZraP, responsible for sensing the presence of external Zn2+. The ZraSR system regulates the expression of zraP, which encodes a periplasmic zinc-binding protein, in response to high concentrations of extracellular zinc ions; phosphorylated ZraR triggers divergent transcription of zraP and zraSR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 907