Difference between revisions of "Part:BBa K4115017"
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The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics. | The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics. | ||
− | [[Image:SacCgrowthcurve1.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E. coli'' with <partinfo>BBa_K4115017</partinfo> in 300ml conical flask in biological shaker, scale: 100 mL M9 medium, 37 °C, 220 rpm, initial pH 7.5, OD<sub>600</sub> measured every 2 hours.]] | + | |
+ | ==Cultivation, Experiment and Improvement Process== | ||
+ | ===100 mL Fermentation ''E. coli'' DH5α with <partinfo>BBa_K4115017</partinfo>=== | ||
+ | [[Image:SacCgrowthcurve1.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E. coli'' with <partinfo>BBa_K4115017</partinfo> in 300ml conical flask in biological shaker, scale: 100 mL M9 medium (with 20% sucrose), 37 °C, 220 rpm, initial pH 7.5, OD<sub>600</sub> measured every 2 hours.]] | ||
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Revision as of 07:13, 3 October 2022
SacC (the extracellular sucrase gene)
The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 437
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1309
Illegal PstI site found at 437 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 437
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 437
Illegal NgoMIV site found at 696
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.
Cultivation, Experiment and Improvement Process
100 mL Fermentation E. coli DH5α with BBa_K4115017