Difference between revisions of "Part:BBa K4387999:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | No codon optimization was necessary. The sequence was taken directly from the reference paper. | + | No codon optimization was necessary. The sequence was taken directly from the reference paper. [1] <partinfo>BBa_K4387987</partinfo> is a composite part, allowing the constitutive expression of the HlyA secretion machinery. |
===Source=== | ===Source=== | ||
| − | |||
Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1] | Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1] | ||
| + | |||
| + | |||
===References=== | ===References=== | ||
* [1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0 | * [1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0 | ||
Revision as of 10:32, 6 October 2022
HlyB of the hemolysin A one-step secretion system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1096
Illegal AgeI site found at 1266 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
No codon optimization was necessary. The sequence was taken directly from the reference paper. [1] BBa_K4387987 is a composite part, allowing the constitutive expression of the HlyA secretion machinery.
Source
Sequence of the HlyB was obtain from the paper of Ruano-Gallego et al. [1]
References
- [1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0